Question 1. What are the three layers of zebrafish epiboly that form three distinct domains? What is the major force driving epiboly?
Question 2. What are the two types of force employed by the actomyosin network to the migration of EVL?
Question 3. Why did the author say the calcium transients are important to epiboly progression? What are the spatial and temporal distributions of calcium during the epiboly?
Question 4. As we discussed in the class, the deep cell movement is regulated by E-cadherin, which is an adhesion molecule maintaining the cell-cell junctions. In the present paper, how many E-cadherin regulators were discussed? What happened if the production of those regulators in zebrafish embryo were reduced?
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